cas9 sequence Search Results


venus  (Addgene inc)
93
Addgene inc venus
Venus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/venus/product/Addgene inc
Average 93 stars, based on 1 article reviews
venus - by Bioz Stars, 2026-03
93/100 stars
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pdd cas9  (Addgene inc)
93
Addgene inc pdd cas9
Pdd Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pdd cas9/product/Addgene inc
Average 93 stars, based on 1 article reviews
pdd cas9 - by Bioz Stars, 2026-03
93/100 stars
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cas9 gene coding sequence  (Epoch Life Signs Inc)
90
Epoch Life Signs Inc cas9 gene coding sequence
Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the <t>Cas9</t> gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.
Cas9 Gene Coding Sequence, supplied by Epoch Life Signs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cas9 gene coding sequence/product/Epoch Life Signs Inc
Average 90 stars, based on 1 article reviews
cas9 gene coding sequence - by Bioz Stars, 2026-03
90/100 stars
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pcc1-orit-cas9 plasmid carrying the corresponding spacer sequence  (SGI-DNA inc)
90
SGI-DNA inc pcc1-orit-cas9 plasmid carrying the corresponding spacer sequence
Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the <t>Cas9</t> gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.
Pcc1 Orit Cas9 Plasmid Carrying The Corresponding Spacer Sequence, supplied by SGI-DNA inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcc1-orit-cas9 plasmid carrying the corresponding spacer sequence/product/SGI-DNA inc
Average 90 stars, based on 1 article reviews
pcc1-orit-cas9 plasmid carrying the corresponding spacer sequence - by Bioz Stars, 2026-03
90/100 stars
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crispr/cas9  (Epigenomics ag)
90
Epigenomics ag crispr/cas9
Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the <t>Cas9</t> gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.
Crispr/Cas9, supplied by Epigenomics ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr/cas9/product/Epigenomics ag
Average 90 stars, based on 1 article reviews
crispr/cas9 - by Bioz Stars, 2026-03
90/100 stars
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btn3a sequences targeted by cr/cas9  (Biotechnology Information)
90
Biotechnology Information btn3a sequences targeted by cr/cas9
Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the <t>Cas9</t> gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.
Btn3a Sequences Targeted By Cr/Cas9, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/btn3a sequences targeted by cr/cas9/product/Biotechnology Information
Average 90 stars, based on 1 article reviews
btn3a sequences targeted by cr/cas9 - by Bioz Stars, 2026-03
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oligonucleotides encoding cas9-target sequences on exon 2 or exon 8 of the murine camk2b gene  (Microsynth ag)
90
Microsynth ag oligonucleotides encoding cas9-target sequences on exon 2 or exon 8 of the murine camk2b gene
Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the <t>Cas9</t> gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.
Oligonucleotides Encoding Cas9 Target Sequences On Exon 2 Or Exon 8 Of The Murine Camk2b Gene, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligonucleotides encoding cas9-target sequences on exon 2 or exon 8 of the murine camk2b gene/product/Microsynth ag
Average 90 stars, based on 1 article reviews
oligonucleotides encoding cas9-target sequences on exon 2 or exon 8 of the murine camk2b gene - by Bioz Stars, 2026-03
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long-read sequencing with crispr/cas9-mediated target selection  (Oxford Nanopore)
90
Oxford Nanopore long-read sequencing with crispr/cas9-mediated target selection
Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the <t>Cas9</t> gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.
Long Read Sequencing With Crispr/Cas9 Mediated Target Selection, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/long-read sequencing with crispr/cas9-mediated target selection/product/Oxford Nanopore
Average 90 stars, based on 1 article reviews
long-read sequencing with crispr/cas9-mediated target selection - by Bioz Stars, 2026-03
90/100 stars
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cas9 coding sequence  (Taihe Biotechnology Co Ltd)
90
Taihe Biotechnology Co Ltd cas9 coding sequence
Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the <t>Cas9</t> gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.
Cas9 Coding Sequence, supplied by Taihe Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cas9 coding sequence/product/Taihe Biotechnology Co Ltd
Average 90 stars, based on 1 article reviews
cas9 coding sequence - by Bioz Stars, 2026-03
90/100 stars
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sequence p414-tef1p-cas9-cyc1t  (Bioneer Corporation)
90
Bioneer Corporation sequence p414-tef1p-cas9-cyc1t
Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the <t>Cas9</t> gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.
Sequence P414 Tef1p Cas9 Cyc1t, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sequence p414-tef1p-cas9-cyc1t/product/Bioneer Corporation
Average 90 stars, based on 1 article reviews
sequence p414-tef1p-cas9-cyc1t - by Bioz Stars, 2026-03
90/100 stars
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cas9 cdna  (Eurofins Genomics)
90
Eurofins Genomics cas9 cdna
Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the <t>Cas9</t> gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.
Cas9 Cdna, supplied by Eurofins Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cas9 cdna/product/Eurofins Genomics
Average 90 stars, based on 1 article reviews
cas9 cdna - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the Cas9 gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.

Journal: Scientific Reports

Article Title: Generation of Trichoderma harzianum with pyr4 auxotrophic marker by using the CRISPR/Cas9 system

doi: 10.1038/s41598-020-80186-4

Figure Lengend Snippet: Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the Cas9 gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.

Article Snippet: For the construction of the CRISPR/Cas9 editing system, the Cas9 gene coding sequence from S. pyogenes was codon-optimized for expression in Trichoderma harzianum and synthesized by Epoch Life Science, Inc. (Sugar Land, TX, USA) ( ).

Techniques: Isolation, Amplification, Sequencing